An allele specific PCR assay for screening for drug resistance among Wuchereria bancrofti populations in India.
Identifieur interne : 006407 ( Main/Exploration ); précédent : 006406; suivant : 006408An allele specific PCR assay for screening for drug resistance among Wuchereria bancrofti populations in India.
Auteurs : S L Hoti [Inde] ; R. Dhamodharan ; K. Subramaniyan ; P K DasSource :
- The Indian journal of medical research [ 0971-5916 ] ; 2009.
Descripteurs français
- KwdFr :
- Albendazole (pharmacologie), Albendazole (usage thérapeutique), Alignement de séquences, Allèles, Animaux, Données de séquences moléculaires, Filaricides (pharmacologie), Filaricides (usage thérapeutique), Filariose lymphatique (parasitologie), Filariose lymphatique (traitement médicamenteux), Humains, Polymorphisme génétique, Réaction de polymérisation en chaîne (), Résistance aux substances (génétique), Séquence d'acides aminés, Séquence nucléotidique, Tests de sensibilité parasitaire, Tubuline (génétique), Wuchereria bancrofti (), Wuchereria bancrofti (génétique), Wuchereria bancrofti (physiologie).
- MESH :
- génétique : Résistance aux substances, Tubuline, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- pharmacologie : Albendazole, Filaricides.
- physiologie : Wuchereria bancrofti.
- traitement médicamenteux : Filariose lymphatique.
- usage thérapeutique : Albendazole, Filaricides.
- Alignement de séquences, Allèles, Animaux, Données de séquences moléculaires, Humains, Polymorphisme génétique, Réaction de polymérisation en chaîne, Séquence d'acides aminés, Séquence nucléotidique, Tests de sensibilité parasitaire, Wuchereria bancrofti.
English descriptors
- KwdEn :
- Albendazole (pharmacology), Albendazole (therapeutic use), Alleles, Amino Acid Sequence, Animals, Base Sequence, Drug Resistance (genetics), Elephantiasis, Filarial (drug therapy), Elephantiasis, Filarial (parasitology), Filaricides (pharmacology), Filaricides (therapeutic use), Humans, Molecular Sequence Data, Parasitic Sensitivity Tests, Polymerase Chain Reaction (methods), Polymorphism, Genetic, Sequence Alignment, Tubulin (genetics), Wuchereria bancrofti (drug effects), Wuchereria bancrofti (genetics), Wuchereria bancrofti (physiology).
- MESH :
- chemical , genetics : Tubulin.
- chemical , pharmacology : Albendazole, Filaricides.
- chemical , therapeutic use : Albendazole, Filaricides.
- drug effects : Wuchereria bancrofti.
- drug therapy : Elephantiasis, Filarial.
- genetics : Drug Resistance, Wuchereria bancrofti.
- methods : Polymerase Chain Reaction.
- parasitology : Elephantiasis, Filarial.
- physiology : Wuchereria bancrofti.
- Alleles, Amino Acid Sequence, Animals, Base Sequence, Humans, Molecular Sequence Data, Parasitic Sensitivity Tests, Polymorphism, Genetic, Sequence Alignment.
Abstract
Albendazole, a commonly used anthelminthic drug that targets the polymerization of alpha- and beta-tubulin dimer is currently co-administered with the antifilarial drug, diethylcarbamazine citrate (DEC) in the ongoing Global Programme for Elimination of Lymphatic Filariasis (GPELF). The experience in veterinary field has shown that there can be a rapid development of resistance to this drug, which therefore, needs to be monitored regularly in GPELF. Hence, we investigated the nucleotide polymorphism in the albendazole-binding domain of the isotype 1 beta-tubulin gene from several populations of Wuchereria bancrofti and developed an AS-PCR assay useful in screening for sensitive/resistance alleles among parasite populations and also evaluated its utility.
PubMed: 19797818
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002D28
- to stream PubMed, to step Curation: 002D28
- to stream PubMed, to step Checkpoint: 002D28
- to stream Ncbi, to step Merge: 003646
- to stream Ncbi, to step Curation: 003646
- to stream Ncbi, to step Checkpoint: 003646
- to stream Main, to step Merge: 006485
- to stream Main, to step Curation: 006407
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An allele specific PCR assay for screening for drug resistance among Wuchereria bancrofti populations in India.</title><author><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name><affiliation wicri:level="1"><nlm:affiliation>Vector Control Research Centre (ICMR), Puducherry, India. slhoti@yahoo.com</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Vector Control Research Centre (ICMR), Puducherry</wicri:regionArea><wicri:noRegion>Puducherry</wicri:noRegion></affiliation></author><author><name sortKey="Dhamodharan, R" sort="Dhamodharan, R" uniqKey="Dhamodharan R" first="R" last="Dhamodharan">R. Dhamodharan</name></author><author><name sortKey="Subramaniyan, K" sort="Subramaniyan, K" uniqKey="Subramaniyan K" first="K" last="Subramaniyan">K. Subramaniyan</name></author><author><name sortKey="Das, P K" sort="Das, P K" uniqKey="Das P" first="P K" last="Das">P K Das</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19797818</idno><idno type="pmid">19797818</idno><idno type="wicri:Area/PubMed/Corpus">002D28</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D28</idno><idno type="wicri:Area/PubMed/Curation">002D28</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002D28</idno><idno type="wicri:Area/PubMed/Checkpoint">002D28</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D28</idno><idno type="wicri:Area/Ncbi/Merge">003646</idno><idno type="wicri:Area/Ncbi/Curation">003646</idno><idno type="wicri:Area/Ncbi/Checkpoint">003646</idno><idno type="wicri:doubleKey">0971-5916:2009:Hoti S:an:allele:specific</idno><idno type="wicri:Area/Main/Merge">006485</idno><idno type="wicri:Area/Main/Curation">006407</idno><idno type="wicri:Area/Main/Exploration">006407</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An allele specific PCR assay for screening for drug resistance among Wuchereria bancrofti populations in India.</title><author><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name><affiliation wicri:level="1"><nlm:affiliation>Vector Control Research Centre (ICMR), Puducherry, India. slhoti@yahoo.com</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Vector Control Research Centre (ICMR), Puducherry</wicri:regionArea><wicri:noRegion>Puducherry</wicri:noRegion></affiliation></author><author><name sortKey="Dhamodharan, R" sort="Dhamodharan, R" uniqKey="Dhamodharan R" first="R" last="Dhamodharan">R. Dhamodharan</name></author><author><name sortKey="Subramaniyan, K" sort="Subramaniyan, K" uniqKey="Subramaniyan K" first="K" last="Subramaniyan">K. Subramaniyan</name></author><author><name sortKey="Das, P K" sort="Das, P K" uniqKey="Das P" first="P K" last="Das">P K Das</name></author></analytic><series><title level="j">The Indian journal of medical research</title><idno type="ISSN">0971-5916</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Albendazole (pharmacology)</term><term>Albendazole (therapeutic use)</term><term>Alleles</term><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Drug Resistance (genetics)</term><term>Elephantiasis, Filarial (drug therapy)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Filaricides (pharmacology)</term><term>Filaricides (therapeutic use)</term><term>Humans</term><term>Molecular Sequence Data</term><term>Parasitic Sensitivity Tests</term><term>Polymerase Chain Reaction (methods)</term><term>Polymorphism, Genetic</term><term>Sequence Alignment</term><term>Tubulin (genetics)</term><term>Wuchereria bancrofti (drug effects)</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Albendazole (pharmacologie)</term><term>Albendazole (usage thérapeutique)</term><term>Alignement de séquences</term><term>Allèles</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Filaricides (pharmacologie)</term><term>Filaricides (usage thérapeutique)</term><term>Filariose lymphatique (parasitologie)</term><term>Filariose lymphatique (traitement médicamenteux)</term><term>Humains</term><term>Polymorphisme génétique</term><term>Réaction de polymérisation en chaîne ()</term><term>Résistance aux substances (génétique)</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Tests de sensibilité parasitaire</term><term>Tubuline (génétique)</term><term>Wuchereria bancrofti ()</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Tubulin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Albendazole</term><term>Filaricides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Albendazole</term><term>Filaricides</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drug Resistance</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Résistance aux substances</term><term>Tubuline</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Albendazole</term><term>Filaricides</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Albendazole</term><term>Filaricides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Humans</term><term>Molecular Sequence Data</term><term>Parasitic Sensitivity Tests</term><term>Polymorphism, Genetic</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Allèles</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Polymorphisme génétique</term><term>Réaction de polymérisation en chaîne</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Tests de sensibilité parasitaire</term><term>Wuchereria bancrofti</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Albendazole, a commonly used anthelminthic drug that targets the polymerization of alpha- and beta-tubulin dimer is currently co-administered with the antifilarial drug, diethylcarbamazine citrate (DEC) in the ongoing Global Programme for Elimination of Lymphatic Filariasis (GPELF). The experience in veterinary field has shown that there can be a rapid development of resistance to this drug, which therefore, needs to be monitored regularly in GPELF. Hence, we investigated the nucleotide polymorphism in the albendazole-binding domain of the isotype 1 beta-tubulin gene from several populations of Wuchereria bancrofti and developed an AS-PCR assay useful in screening for sensitive/resistance alleles among parasite populations and also evaluated its utility.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Das, P K" sort="Das, P K" uniqKey="Das P" first="P K" last="Das">P K Das</name><name sortKey="Dhamodharan, R" sort="Dhamodharan, R" uniqKey="Dhamodharan R" first="R" last="Dhamodharan">R. Dhamodharan</name><name sortKey="Subramaniyan, K" sort="Subramaniyan, K" uniqKey="Subramaniyan K" first="K" last="Subramaniyan">K. Subramaniyan</name></noCountry><country name="Inde"><noRegion><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006407 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006407 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19797818
|texte= An allele specific PCR assay for screening for drug resistance among Wuchereria bancrofti populations in India.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19797818" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |